pGL3con-miR15aBul
(Plasmid
#21117)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21117 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGL3-control
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5256
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehsa-miR-15a sensor
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SpeciesSequences containing three hsa-miR-15a complementary sites (with mismatches)
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Insert Size (bp)90
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (destroyed during cloning)
- 3′ cloning site XbaI (destroyed during cloning)
- 5′ sequencing primer GTACCGAAAGGTCTTACCG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL3con-miR15aBul was a gift from Joshua Mendell (Addgene plasmid # 21117 ; http://n2t.net/addgene:21117 ; RRID:Addgene_21117) -
For your References section:
Cell-cell contact globally activates microRNA biogenesis. Hwang HW, Wentzel EA, Mendell JT. Proc Natl Acad Sci U S A. 2009 Apr 9. ():. 10.1073/pnas.0811523106 PubMed 19359480
