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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 21115 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+) (Invitrogen)
- Backbone size w/o insert (bp) 5428
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemiR-26a2
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SpeciesH. sapiens (human)
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Insert Size (bp)313
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Entrez GeneMIR26A2 (a.k.a. MIRN26A2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-miR26a2 was a gift from Joshua Mendell (Addgene plasmid # 21115 ; http://n2t.net/addgene:21115 ; RRID:Addgene_21115) -
For your References section:
Cell-cell contact globally activates microRNA biogenesis. Hwang HW, Wentzel EA, Mendell JT. Proc Natl Acad Sci U S A. 2009 Apr 9. ():. 10.1073/pnas.0811523106 PubMed 19359480