pcDNA3-miR17
(Plasmid #21113)

• Depositing Lab: Joshua Mendell
• Publication: Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 9. ():.

Backbone

• Vector backbone: pcDNA3.1(+) (Invitrogen)
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: miR-17
• Species: H. sapiens (human)
• Insert Size (bp): 286
• Entrez Gene: MIR17 (a.k.a. MIR17-5p, MIR91, MIRN17, MIRN91, hsa-mir-17, miR-17, miR17-3p, miRNA17, miRNA91)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry