Skip to main content
Addgene

pUC19-Top2A-5X Gly-Venus-T2A-Hygro
(Plasmid #211115)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211115 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pUC19
  • Backbone manufacturer
    ThermoFisher
  • Backbone size w/o insert (bp) 2686
  • Total vector size (bp) 6562
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression, Bacterial Expression, CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    5' C-term Top2A homology arm
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    800
  • GenBank ID
    NG_027678.1
  • Entrez Gene
    TOP2A (a.k.a. TOP2, TOP2alpha, TOPIIA, TP2A)
  • Promoter n/a

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Venus
  • Species
    Aequorea victoria
  • Insert Size (bp)
    720
  • Promoter n/a

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    T2A
  • Species
    Synthetic
  • Insert Size (bp)
    57
  • Promoter n/a

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    Blastocidin Resistance
  • Species
    Bacillus cereus
  • Insert Size (bp)
    987
  • Promoter n/a

Cloning Information for Gene/Insert 4

Gene/Insert 5

  • Gene/Insert name
    5' C-term Top2A homology arm
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    800
  • GenBank ID
    NG_027678.1
  • Entrez Gene
    TOP2A (a.k.a. TOP2, TOP2alpha, TOPIIA, TP2A)
  • Promoter n/a

Cloning Information for Gene/Insert 5

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19-Top2A-5X Gly-Venus-T2A-Hygro was a gift from Job Dekker (Addgene plasmid # 211115 ; http://n2t.net/addgene:211115 ; RRID:Addgene_211115)

  • For your References section:

    Mitotic chromosomes are self-entangled and disentangle through a topoisomerase-II-dependent two-stage exit from mitosis. Hildebrand EM, Polovnikov K, Dekker B, Liu Y, Lafontaine DL, Fox AN, Li Y, Venev SV, Mirny LA, Dekker J. Mol Cell. 2024 Mar 13:S1097-2765(24)00144-8. doi: 10.1016/j.molcel.2024.02.025. 10.1016/j.molcel.2024.02.025 PubMed 38521067
Related items: