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pcDNA3.1/V5-His-TOPO-mir17-92
(Plasmid #21109)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21109 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1/V5-His-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5523
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    miR-17-92 cluster
  • Alt name
    miR17 cluster
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1117
  • Entrez Gene
    MIR17 (a.k.a. MIR17-5p, MIR91, MIRN17, MIRN91, hsa-mir-17, miR-17, miR17-3p, miRNA17, miRNA91)

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ cloning site TOPO (not destroyed)
  • 3′ cloning site TOPO (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1/V5-His-TOPO-mir17-92 was a gift from Joshua Mendell (Addgene plasmid # 21109 ; http://n2t.net/addgene:21109 ; RRID:Addgene_21109)

  • For your References section:

    c-Myc-regulated microRNAs modulate E2F1 expression. O'Donnell KA, Wentzel EA, Zeller KI, Dang CV, Mendell JT. Nature. 2005 Jun 9. 435(7043):839-43. 10.1038/nature03677 PubMed 15944709
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