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PBS/pU6-HIF1 alpha RNAi plasmid 2
(Plasmid #21104)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21104 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PBS/U6
  • Backbone size w/o insert (bp) 3300
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RNAi against hypoxia inducible factor 1alpha
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    70

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site APAI (destroyed during cloning)
  • 3′ cloning site ECORI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HIF1α RNAi target sequence:
GGGTTGAAACTCAAGCAACTG.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PBS/pU6-HIF1 alpha RNAi plasmid 2 was a gift from Connie Cepko (Addgene plasmid # 21104 ; http://n2t.net/addgene:21104 ; RRID:Addgene_21104)

  • For your References section:

    HDAC4 regulates neuronal survival in normal and diseased retinas. Chen B, Cepko CL. Science. 2009 Jan 9. 323(5911):256-9. 10.1126/science.1166226 PubMed 19131628
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