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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21102 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAGEN
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHYPOXIA INDUCIBLE FACTOR 1 ALPHA-K532R MUTANT
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Alt nameHIF1A
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SpeciesH. sapiens (human)
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Insert Size (bp)2400
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Entrez GeneHIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NOT1 (not destroyed)
- 3′ cloning site NOT1 (not destroyed)
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byK.W. Kim, Seoul National University
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-HIF1 alpha K532R was a gift from Connie Cepko (Addgene plasmid # 21102 ; http://n2t.net/addgene:21102 ; RRID:Addgene_21102) -
For your References section:
HDAC4 regulates neuronal survival in normal and diseased retinas. Chen B, Cepko CL. Science. 2009 Jan 9. 323(5911):256-9. 10.1126/science.1166226 PubMed 19131628