pCAG-HIF1 alpha
(Plasmid #21101)

• Depositing Lab: Connie Cepko
• Publication: Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Backbone

• Vector backbone: pCAGEN
• Backbone manufacturer: Addgene Plasmid 11160
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HYPOXIA INDUCIBLE FACTOR 1ALPHA
• Alt name: HIF1A
• Species: H. sapiens (human)
• Insert Size (bp): 2400
• Entrez Gene: HIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NOT1 (not destroyed)
• 3′ cloning site: NOT1 (not destroyed)
• 5′ sequencing primer: pCAG-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: K.W. Kim, Seoul National University
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Compared to GenBank reference sequence NP_001521.1 the HIF1 alpha insert in this plasmid contains C217Y & A279D mutations.