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pCAG-hHDAC4
(Plasmid #21096)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21096 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGEN
  • Backbone manufacturer
    Cepko lab
  • Backbone size w/o insert (bp) 4798
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Histone Deacetylase 4
  • Alt name
    HDAC4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3300
  • Mutation
    shRNA target sequence was mutated (mutated nucleotides in small letters) to GGAaATGCTaGCtATGAAaCA using degenerate codons
  • Entrez Gene
    HDAC4 (a.k.a. AHO3, BDMR, HA6116, HD4, HDAC-4, HDAC-A, HDACA, NEDCHF, NEDCHID)
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site Not1 (destroyed during cloning)
  • 5′ sequencing primer pCAG-F
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    J.K Lu University of Florida

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

WILD TYPE HDAC4
PROTEIN WITH DEGENERATE CODONS
SCIENCE. 2009 JAN 09; 323 (5911):256-9.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-hHDAC4 was a gift from Connie Cepko (Addgene plasmid # 21096 ; http://n2t.net/addgene:21096 ; RRID:Addgene_21096)

  • For your References section:

    HDAC4 regulates neuronal survival in normal and diseased retinas. Chen B, Cepko CL. Science. 2009 Jan 9. 323(5911):256-9. 10.1126/science.1166226 PubMed 19131628
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