CBP-HAT-GST
(Plasmid #21093)

• Depositing Lab: Corey Largman
• Publication: Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Backbone

• Vector backbone: pGEX-2T
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 4948
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CBP
• Species: M. musculus (mouse)
• Insert Size (bp): 2337
• Mutation: HAT domain: aa 1196-1718
• Entrez Gene: Crebbp (a.k.a. CBP, CBP/p300, KAT3A, p300/CBP)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pGEX-5
• 3′ sequencing primer: pGEX-3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: R. Goodman and T. Kouzarides
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The protein product of this clone exhibits good HAT activity on histone substrates.