CBP-HAT-pET
(Plasmid #21091)

• Depositing Lab: Corey Largman
• Publication: Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Backbone

• Vector backbone: pET
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CBP
• Species: M. musculus (mouse)
• Insert Size (bp): 1347
• Mutation: HAT domain aa 1171-1621
• Entrez Gene: Crebbp (a.k.a. CBP, CBP/p300, KAT3A, p300/CBP)
• Tags / Fusion Proteins:
  • His (N terminal on backbone)
  • T7 tag (N terminal on backbone)
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Resource Information

• A portion of this plasmid was derived from a plasmid made by: R. Goodman and T. Kouzarides

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry