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Addgene

CBP-HAT-SP65
(Plasmid #21088)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21088 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP65
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3000
  • Vector type
    in vitro TNT

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CBP
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1347
  • Mutation
    HAT domain aa 1172-1621
  • Entrez Gene
    Crebbp (a.k.a. CBP, CBP/p300, KAT3A, p300/CBP)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer M13_rev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    R. Goodman and T. Kouzarides

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CBP-HAT-SP65 was a gift from Corey Largman (Addgene plasmid # 21088 ; http://n2t.net/addgene:21088 ; RRID:Addgene_21088)

  • For your References section:

    The HOX homeodomain proteins block CBP histone acetyltransferase activity. Shen WF, Krishnan K, Lawrence HJ, Largman C. Mol Cell Biol. 2001 Nov;21(21):7509-22 10.1128/MCB.21.21.7509-7522.2001 PubMed 11585930
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