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Addgene

HOXB4-FLAG-WG-SP65
(Plasmid #21087)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21087 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP65
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3000
  • Vector type
    In vitro T/T

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXB4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1100
  • Mutation
    W to G mutation in YPWM Pbx1 interaction motif, protein does not bind Pbx1
  • Entrez Gene
    HOXB4 (a.k.a. HOX-2.6, HOX2, HOX2F)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (blunted) (destroyed during cloning)
  • 3′ cloning site HindIII (blunted) (destroyed during cloning)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer M13-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PBX interaction mutant

Please note that though the author's map suggests the insert is FLAG-tagged, Addgene was unable to confirm the presence of the FLAG.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXB4-FLAG-WG-SP65 was a gift from Corey Largman (Addgene plasmid # 21087 ; http://n2t.net/addgene:21087 ; RRID:Addgene_21087)

  • For your References section:

    Hox homeodomain proteins exhibit selective complex stabilities with Pbx and DNA. Shen WF, Chang CP, Rozenfeld S, Sauvageau G, Humphries RK, Lu M, Lawrence HJ, Cleary ML, Largman C.. Nucleic Acids Res. 1996 Mar 1;24(5):898-906.Click here to read 10.1093/nar/24.5.898 PubMed 8600458
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