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Addgene

pJTM-T7-mNeongreen-6xHis
(Plasmid #210868)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210868 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mNeongreen-6xHistag
  • Promoter T7

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TTTAACTTTAAGAAGGAGATATAATGGTCTCCAAGGGCGAGG
  • 3′ sequencing primer TTTTTTGGCTGAATTAATGGTGATGGTGATGGTGCTTATAGAGCTCATCCATCCCCATCA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJTM-T7-mNeongreen-6xHis was a gift from Richard Murray (Addgene plasmid # 210868 ; http://n2t.net/addgene:210868 ; RRID:Addgene_210868)

  • For your References section:

    Development of Cell-Free Transcription-Translation Systems in Three Soil Pseudomonads. Meyerowitz JT, Larsson EM, Murray RM. ACS Synth Biol. 2024 Feb 16;13(2):530-537. doi: 10.1021/acssynbio.3c00468. Epub 2024 Feb 6. 10.1021/acssynbio.3c00468 PubMed 38319019
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