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pBbA5k-AcrAB-AsLOV2*(543)
(Plasmid #210863)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210863 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBbA5k
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    acrA
  • Species
    E. coli
  • Insert Size (bp)
    1194
  • GenBank ID
    NP_414996.1

Gene/Insert 2

  • Gene/Insert name
    acrB
  • Species
    E. coli
  • Insert Size (bp)
    3147
  • GenBank ID
    NP_414995.1
  • Tag / Fusion Protein
    • AsLOV2*(543) (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBbA5k-AcrAB-AsLOV2*(543) was a gift from Mary Dunlop (Addgene plasmid # 210863 ; http://n2t.net/addgene:210863 ; RRID:Addgene_210863)

  • For your References section:

    Light-inducible protein degradation in E. coli with the LOVdeg tag. Tague N, Coriano-Ortiz C, Sheets MB, Dunlop MJ. Elife. 2024 Jan 25;12:RP87303. doi: 10.7554/eLife.87303. 10.7554/eLife.87303 PubMed 38270583
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