pCAG-GST-ATG13 (363-450)
(Plasmid
#210849)
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PurposeMammalian expression of ATG13 (363-450) mutant
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 210849 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATG13
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SpeciesH. sapiens (human)
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Entrez GeneATG13 (a.k.a. KIAA0652, PARATARG8)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.06.01.543278v3 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-GST-ATG13 (363-450) was a gift from James Hurley (Addgene plasmid # 210849 ; http://n2t.net/addgene:210849 ; RRID:Addgene_210849) -
For your References section:
Structure and activation of the human autophagy-initiating ULK1C:PI3KC3-C1 supercomplex. Chen M, Ren X, Nguyen TN, Khuu G, Cook ASI, Lazarou M, Hurley JH. bioRxiv 2023.06.01.543278 10.1101/2023.06.01.543278