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Addgene

htrpC6-YFP
(Plasmid #21084)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21084 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Toxic to E. coli
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TrpC6
  • Species
    H. sapiens (human)
  • Entrez Gene
    TRPC6 (a.k.a. FSGS2, TRP6)
  • Tag / Fusion Protein
    • YFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    htrpC6-YFP was a gift from Craig Montell (Addgene plasmid # 21084 ; http://n2t.net/addgene:21084 ; RRID:Addgene_21084)

  • For your References section:

    Integration of phosphoinositide- and calmodulin-mediated regulation of TRPC6. Kwon Y, Hofmann T, Montell C. Mol Cell. 2007 Feb 23. 25(4):491-503. 10.1016/j.molcel.2007.01.021 PubMed 17317623
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