mTALEAD 16S rRNA-1139T10C
(Plasmid
#210838)
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PurposemTALEAD expression vector
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 210838 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepK7WG4
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFor specific A-to-G base editing to introduce targeted A-to-G mutation in 16S rRNA in chloroplast
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SpeciesSynthetic
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Ti vector constructed by multisite Gateway
Please note: Plasmid contains a K214N mutation and Leu insertion after G826 in the mTALEAD ORF which are not known to affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mTALEAD 16S rRNA-1139T10C was a gift from Shin-ichi Arimura (Addgene plasmid # 210838 ; http://n2t.net/addgene:210838 ; RRID:Addgene_210838) -
For your References section:
Targeted A-to-G base editing in the organellar genomes of Arabidopsis with monomeric programmable deaminases. Zhou C, Okuno M, Nakazato I, Tsutsumi N, Arimura SI. Plant Physiol. 2023 Dec 21:kiad678. doi: 10.1093/plphys/kiad678. 10.1093/plphys/kiad678 PubMed 38128544
