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mTALEAD 16S rRNA-1139T8C
(Plasmid #210837)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210837 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pK7WG3
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    For specific A-to-G base editing to introduce targeted A-to-G mutation in 16S rRNA in chloroplast
  • Species
    Synthetic

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Ti vector constructed by multisite Gateway

Please note: Plasmid contains a K214N mutation, E660-P692 deletion, and HD701-702NI mutations in the mitoTALE ORF. These mutations are not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mTALEAD 16S rRNA-1139T8C was a gift from Shin-ichi Arimura (Addgene plasmid # 210837 ; http://n2t.net/addgene:210837 ; RRID:Addgene_210837)

  • For your References section:

    Targeted A-to-G base editing in the organellar genomes of Arabidopsis with monomeric programmable deaminases. Zhou C, Okuno M, Nakazato I, Tsutsumi N, Arimura SI. Plant Physiol. 2023 Dec 21:kiad678. doi: 10.1093/plphys/kiad678. 10.1093/plphys/kiad678 PubMed 38128544
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