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pET28sumo-hRig-I DCARDS
(Plasmid #210828)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210828 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pet28-sumo
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hRIG-I DCARDS (DDX58) with Caspase Recruitment domains deleted
  • Species
    H. sapiens (human)
  • Mutation
    NA
  • Entrez Gene
    RIGI (a.k.a. DDX58, RIG-I, RIG1, RLR-1, SGMRT2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28sumo-hRig-I DCARDS was a gift from Anna Pyle (Addgene plasmid # 210828 ; http://n2t.net/addgene:210828 ; RRID:Addgene_210828)

  • For your References section:

    Structural insights into RNA recognition by RIG-I. Luo D, Ding SC, Vela A, Kohlway A, Lindenbach BD, Pyle AM. Cell. 2011 Oct 14;147(2):409-22. doi: 10.1016/j.cell.2011.09.023. 10.1016/j.cell.2011.09.023 PubMed 22000018
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