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Addgene

pET28sumo-MDA5-Full-length
(Plasmid #210826)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210826 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pet28-sumo
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MDA5 (IFIH1) Full Length Protein
  • Species
    H. sapiens (human)
  • Mutation
    NA
  • Entrez Gene
    IFIH1 (a.k.a. AGS7, Hlcd, IDDM19, IMD95, MDA-5, MDA5, RLR-2, SGMRT1)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28sumo-MDA5-Full-length was a gift from Anna Pyle (Addgene plasmid # 210826 ; http://n2t.net/addgene:210826 ; RRID:Addgene_210826)

  • For your References section:

    Dicer-related helicase 3 forms an obligate dimer for recognizing 22G-RNA. Fitzgerald ME, Vela A, Pyle AM. Nucleic Acids Res. 2014 Apr;42(6):3919-30. doi: 10.1093/nar/gkt1383. Epub 2014 Jan 15. 10.1093/nar/gkt1383 PubMed 24435798
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