pET28a-mTagBFP2-TEV-Strep-His
(Plasmid #210824)

• Purpose: Bacterial overexpression vector for C-terminally Strep and His tagged mTagBFP2 fluorescent protein
• Depositing Lab: Luke Chao
• Publication: Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7554/eLife.100061.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5228
• Total vector size (bp): 6116
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mTagBFP2
• Species: Synthetic
• Insert Size (bp): 888
• Promoter: T7
• Tag / Fusion Protein:
  • TwinStrep, 10xHis (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7-F
• 3′ sequencing primer: T7 Term-R

Resource Information

• Supplemental Documents:
  • pTAB-062_mTagBFP2_StrepHis.gb
• A portion of this plasmid was derived from a plasmid made by: Michael Davidson (Addgene # 54572)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2023.11.08.566258 for bioRxiv preprint.