pCS2-HsProm1s1-StayGold
(Plasmid #210821)

• Purpose: Mammalian overexpression vector for C-terminally StayGold tagged human Prom1s1 (WT)
• Depositing Lab: Luke Chao
• Publication: Bell et al bioRxiv 2023.11.08.566258

Backbone

• Vector backbone: pCS2
• Backbone manufacturer: Nathan D. Lawson
• Backbone size w/o insert (bp): 4072
• Total vector size (bp): 7333
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Prominin 1
• Alt name: PROM1
• Species: H. sapiens (human)
• Insert Size (bp): 3262
• Entrez Gene: PROM1 (a.k.a. AC133, CD133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4)
• Promoter: CMV
• Tag / Fusion Protein:
  • StayGold (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • pTAB-100_pCS2-Prom1s1-StayGold-TWT.gb
• A portion of this plasmid was derived from a plasmid made by: Modified version of construct from Noriaki Sasai (Nara Institute of Science and Technology).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2023.11.08.566258 for bioRxiv preprint.

Original construct published in: Hori, A., Nishide, K., Yasukuni, Y., Haga, K., Kakuta, W., Ishikawa, Y., Hayes, M.J., Ohnuma, S., Kiyonari, H., Kimura, K., et al. (2019). Prominin-1 Modulates Rho/ROCK-Mediated Membrane Morphology and Calcium-Dependent Intracellular Chloride Flux. Sci. Rep. 9, 15911. 10.1038/s41598-019-52040-9.