pcDNA3 N-MLSN
(Plasmid
#21082)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21082 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5446
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTRPM1
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Alt nameMLSN-S
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SpeciesH. sapiens (human)
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Insert Size (bp)1500
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Entrez GeneTRPM1 (a.k.a. CSNB1C, LTRPC1, MLSN1)
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Tag
/ Fusion Protein
- Myc (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3 N-MLSN was a gift from Craig Montell (Addgene plasmid # 21082 ; http://n2t.net/addgene:21082 ; RRID:Addgene_21082) -
For your References section:
Regulation of melastatin, a TRP-related protein, through interaction with a cytoplasmic isoform. Xu XZ, Moebius F, Gill DL, Montell C. Proc Natl Acad Sci U S A. 2001 Sep 11. 98(19):10692-7. 10.1073/pnas.191360198 PubMed 11535825