pCDNA3.1-HsPcdh21-mNeonGreen
(Plasmid
#210819)
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PurposeMammalian overexpression vector for C-terminally mNeonGreen tagged human Pchd21 (WT)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 210819 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDNA3.1
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Backbone manufacturerThermo Fisher
- Backbone size w/o insert (bp) 5373
- Total vector size (bp) 8670
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameProtocadherin 21
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Alt namePCDH21
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Alt nameCadherin-related family member 1
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Alt nameCDHR1
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SpeciesH. sapiens (human)
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Insert Size (bp)3297
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Entrez GeneCDHR1 (a.k.a. CORD15, PCDH21, PRCAD, RP65)
- Promoter CMV
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Tag
/ Fusion Protein
- mNeonGreen (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.11.08.566258 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3.1-HsPcdh21-mNeonGreen was a gift from Luke Chao (Addgene plasmid # 210819 ; http://n2t.net/addgene:210819 ; RRID:Addgene_210819) -
For your References section:
Prominin 1 and Tweety Homology 1 both induce extracellular vesicle formation. Bell TA, Luce BE, Hakim P, Ananda VY, Dardari H, Nguyen TH, Monshizadeh A, Chao LH. bioRxiv 2023.11.08.566258 10.1101/2023.11.08.566258