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pCDNA3.1-HsPcdh21-Myc-Flag-His
(Plasmid #210818)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210818 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1
  • Backbone manufacturer
    Thermo Fisher
  • Backbone size w/o insert (bp) 5373
  • Total vector size (bp) 8163
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Protocadherin 21
  • Alt name
    PCDH21
  • Alt name
    Cadherin-related family member 1
  • Alt name
    CDHR1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2790
  • Entrez Gene
    CDHR1 (a.k.a. CORD15, PCDH21, PRCAD, RP65)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc, 3x Flag, 10x His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.11.08.566258 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3.1-HsPcdh21-Myc-Flag-His was a gift from Luke Chao (Addgene plasmid # 210818 ; http://n2t.net/addgene:210818 ; RRID:Addgene_210818)

  • For your References section:

    Prominin 1 and Tweety Homology 1 both induce extracellular vesicle formation. Bell TA, Luce BE, Hakim P, Ananda VY, Dardari H, Nguyen TH, Monshizadeh A, Chao LH. Elife. 2024 Aug 13;13:e100061. doi: 10.7554/eLife.100061. 10.7554/eLife.100061 PubMed 39168849
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