pAdEasy-hALDOA
(Plasmid
#210812)
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PurposeTo package adenovirus expressing wild-type aldolaseA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 210812 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAdEasy-1
- Backbone size w/o insert (bp) 33500
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Vector typeAdenoviral
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameALDOA
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SpeciesH. sapiens (human)
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Insert Size (bp)1092
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Entrez GeneALDOA (a.k.a. ALDA, GSD12, HEL-S-87p)
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAdEasy-hALDOA was a gift from Sheng-cai Lin (Addgene plasmid # 210812 ; http://n2t.net/addgene:210812 ; RRID:Addgene_210812) -
For your References section:
Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK. Zhang CS, Hawley SA, Zong Y, Li M, Wang Z, Gray A, Ma T, Cui J, Feng JW, Zhu M, Wu YQ, Li TY, Ye Z, Lin SY, Yin H, Piao HL, Hardie DG, Lin SC. Nature. 2017 Aug 3;548(7665):112-116. doi: 10.1038/nature23275. Epub 2017 Jul 19. 10.1038/nature23275 PubMed 28723898