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Addgene

pUAST-Gr64a
(Plasmid #21081)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21081 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUAST
  • Backbone size w/o insert (bp) 11000
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Gr64a
  • Alt name
    CG32261
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1500
  • Entrez Gene
    Gr64a (a.k.a. CG14986, CG32261, Dmel64a, Dmel\CG32261, Gr64, Gr64A1, GRLU.3, GrLU3, LU.3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site Acc651 (not destroyed)
  • 5′ sequencing primer pCasper-hs
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUAST-Gr64a was a gift from Craig Montell (Addgene plasmid # 21081 ; http://n2t.net/addgene:21081 ; RRID:Addgene_21081)

  • For your References section:

    A Drosophila gustatory receptor required for the responses to sucrose, glucose, and maltose identified by mRNA tagging. Jiao Y, Moon SJ, Montell C. Proc Natl Acad Sci U S A. 2007 Aug 28. 104(35):14110-5. 10.1073/pnas.0702421104 PubMed 17715294
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