FGF8-P2A-iCre
(Plasmid
#210800)
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PurposeRecombinase reporter for FGF8 expression
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 210800 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepl752
- Backbone size w/o insert (bp) 5245
- Total vector size (bp) 8323
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Vector typeMammalian Expression, Cre/Lox
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFGF8
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SpeciesH. sapiens (human)
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Insert Size (bp)3500
- Promoter endougeneous FGF8 promoter
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Tag
/ Fusion Protein
- iCre (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer aattaaccctcactaaagg
- 3′ sequencing primer cctatagtgagtcgtatta (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FGF8-P2A-iCre was a gift from Yuejun Chen (Addgene plasmid # 210800 ; http://n2t.net/addgene:210800 ; RRID:Addgene_210800) -
For your References section:
Mapping of clonal lineages across developmental stages in human neural differentiation. You Z, Wang L, He H, Wu Z, Zhang X, Xue S, Xu P, Hong Y, Xiong M, Wei W, Chen Y. Cell Stem Cell. 2023 Apr 6;30(4):473-487.e9. doi: 10.1016/j.stem.2023.02.007. Epub 2023 Mar 17. 10.1016/j.stem.2023.02.007 PubMed 36933556
Map uploaded by the depositor.
