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Addgene

FGF8-P2A-iCre
(Plasmid #210800)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210800 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pl752
  • Backbone size w/o insert (bp) 5245
  • Total vector size (bp) 8323
  • Vector type
    Mammalian Expression, Cre/Lox
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FGF8
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3500
  • Entrez Gene
    FGF8 (a.k.a. AIGF, FGF-8, HBGF-8, HH6, KAL6)
  • Promoter endougeneous FGF8 promoter
  • Tag / Fusion Protein
    • iCre (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer aattaaccctcactaaagg
  • 3′ sequencing primer cctatagtgagtcgtatta
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FGF8-P2A-iCre was a gift from Yuejun Chen (Addgene plasmid # 210800 ; http://n2t.net/addgene:210800 ; RRID:Addgene_210800)

  • For your References section:

    Mapping of clonal lineages across developmental stages in human neural differentiation. You Z, Wang L, He H, Wu Z, Zhang X, Xue S, Xu P, Hong Y, Xiong M, Wei W, Chen Y. Cell Stem Cell. 2023 Apr 6;30(4):473-487.e9. doi: 10.1016/j.stem.2023.02.007. Epub 2023 Mar 17. 10.1016/j.stem.2023.02.007 PubMed 36933556
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