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Addgene

pUAST-Gr64f
(Plasmid #21080)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21080 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUAST
  • Backbone size w/o insert (bp) 11000
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Gr64f
  • Alt name
    CG32255
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1500
  • Entrez Gene
    Gr64f (a.k.a. CG14988, DmGr64f, Gr64A3, Dmel\CG32255, CG32255)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site Acc651 (not destroyed)
  • 5′ sequencing primer pCasper-hs
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUAST-Gr64f was a gift from Craig Montell (Addgene plasmid # 21080 ; http://n2t.net/addgene:21080 ; RRID:Addgene_21080)

  • For your References section:

    Gr64f is required in combination with other gustatory receptors for sugar detection in Drosophila. Jiao Y, Moon SJ, Wang X, Ren Q, Montell C. Curr Biol. 2008 Nov 25. 18(22):1797-801. 10.1016/j.cub.2008.10.009 PubMed 19026541
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