pCDNA3-SREBP-1 S1P and S2P mutations (U124AFG200-5)
(Plasmid #210767)

• Purpose: Mammalian expression of SREBP-1 (isoform 1) with mutations in residues reported to be critical for S1P and S2P cleavage.
• Depositing Lab: Allan Weissman
• Publication: Yang et al J Biol Chem. 2023 Oct 12;299(12):105346. doi: 10.1016/j.jbc.2023.105346.

Backbone

• Vector backbone: pCDNA3.1(+)
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SREBP-1
• Species: H. sapiens (human)
• Mutation: DRSR 514-517 to AS; 557 R to A; multiple silent mutations to facilitate cloning
• Entrez Gene: SREBF1 (a.k.a. HMD, IFAP2, SREBP1, bHLHd1)
• Tags / Fusion Proteins:
  • T7x2 (N terminal on insert)
  • E-tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nhe1 (not destroyed)
• 3′ cloning site: Not1 (not destroyed)
• 5′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pCDNA3-SREBP-1 S1P and S2P mutations (U124AFG200-5)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Designed with multiple multiple silent mutations to facilitate cloning. Mutations in residues reported to be critical for S1P and S2P cleavage. Starting construct for SREBP-1a plasmids used in PMID: 37838170. Synthesized by GenScript.