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pCDNA3-SREBP-1 S1P and S2P mutations (U124AFG200-5)
(Plasmid #210767)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210767 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1(+)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SREBP-1
  • Species
    H. sapiens (human)
  • Mutation
    DRSR 514-517 to AS; 557 R to A; multiple silent mutations to facilitate cloning
  • Entrez Gene
    SREBF1 (a.k.a. HMD, IFAP2, SREBP1, bHLHd1)
  • Tags / Fusion Proteins
    • T7x2 (N terminal on insert)
    • E-tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Designed with multiple multiple silent mutations to facilitate cloning. Mutations in residues reported to be critical for S1P and S2P cleavage. Starting construct for SREBP-1a plasmids used in PMID: 37838170. Synthesized by GenScript.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3-SREBP-1 S1P and S2P mutations (U124AFG200-5) was a gift from Allan Weissman (Addgene plasmid # 210767 ; http://n2t.net/addgene:210767 ; RRID:Addgene_210767)

  • For your References section:

    SARS-CoV-2 papain-like protease plays multiple roles in regulating cellular proteins in the endoplasmic reticulum. Yang M, Mariano J, Su R, Smith CE, Das S, Gill C, Andresson T, Loncarek J, Tsai YC, Weissman AM. J Biol Chem. 2023 Oct 12;299(12):105346. doi: 10.1016/j.jbc.2023.105346. 10.1016/j.jbc.2023.105346 PubMed 37838170
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