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Addgene

pCambia3301-AtU6-MAR1
(Plasmid #210757)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210757 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCambia3301
  • Vector type
    Plant Expression, CRISPR
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    AtU6-26 pro::MAR1 sgRNA
  • gRNA/shRNA sequence
    CTGGAGTTCTGGACCGTCCT
  • Species
    A. thaliana (mustard weed)
  • Promoter AtU6-26

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCambia3301-AtU6-MAR1 was a gift from Daisuke Miki (Addgene plasmid # 210757 ; http://n2t.net/addgene:210757 ; RRID:Addgene_210757)

  • For your References section:

    Double step screening using endogenous marker improves relative gene targeting efficiency in Arabidopsis. Cheng Y, Zhang L, Ke Y, Dang X, Miki D. Sci Rep. 2024 Dec 28;14(1):30791. doi: 10.1038/s41598-024-80352-y. 10.1038/s41598-024-80352-y PubMed 39730554
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