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pUC57-ITR2-H1- sgCTG Bilbo -eCMV-CjSpD8A-3XFLAG-SV40 NLS-ITR2
(Plasmid #210749)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210749 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Backbone manufacturer
    Genscript
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    CjSp D8A Cas9
  • Species
    Synthetic
  • Mutation
    D8A, N-terminal Cj Cas9, C-terminal Sp Cas9
  • Promoter eCMV
  • Tag / Fusion Protein
    • 3xFLAG, SV40 NLS, PolyA signal (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Bilbo sgCAG
  • Alt name
    gRNA target: GCTGCTGCTGCTGCTGCTGGTTTTAGTCCCTGAAAAGGGACTAAAATGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCT
  • Promoter H1

Cloning Information for Gene/Insert 2

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Genscript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.10.24.563270 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC57-ITR2-H1- sgCTG Bilbo -eCMV-CjSpD8A-3XFLAG-SV40 NLS-ITR2 was a gift from Vincent Dion (Addgene plasmid # 210749 ; http://n2t.net/addgene:210749 ; RRID:Addgene_210749)

  • For your References section:

    Developing small Cas9 hybrids using molecular modeling. Mangin A, Dion V, Menzies G. bioRxiv 2023.10.24.563270 10.1101/2023.10.24.563270
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