AAV-CamK2-tTA
(Plasmid
#210731)
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PurposeAAV vector expressing tetracycline-controlled transactivator (tTA) under CamK2 promoter (for expressing tTA in glutamatergic neurons)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 210731 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV2
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametetracycline-controlled transactivator (tTA)
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SpeciesSynthetic
- Promoter CamK2
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-CamK2-tTA was a gift from Wei Xu (Addgene plasmid # 210731 ; http://n2t.net/addgene:210731 ; RRID:Addgene_210731) -
For your References section:
Brain-wide circuit-specific targeting of astrocytes. Thompson A, Arano R, Saleem U, Preciado R, Munoz L, Nelson I, Ramos K, Kim Y, Li Y, Xu W. Cell Rep Methods. 2023 Dec 18;3(12):100653. doi: 10.1016/j.crmeth.2023.100653. Epub 2023 Dec 4. 10.1016/j.crmeth.2023.100653 PubMed 38052209