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Addgene

NIR-AG
(Plasmid #210728)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210728 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pN1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3813
  • Vector type
    Mammalian Expression
  • Selectable markers
    EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NIR-AG
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6038
  • Entrez Gene
    GSDMD (a.k.a. DF5L, DFNA5L, FKSG10, GSDMDC1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site MunI (unknown if destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    human GSDMD was cloned from Addgene plasmid # 111559

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NIR-AG was a gift from Vladislav Verkhusha (Addgene plasmid # 210728 ; http://n2t.net/addgene:210728 ; RRID:Addgene_210728)

  • For your References section:

    Near-Infrared Optogenetic Module for Conditional Protein Splicing. Karasev MM, Verkhusha VV, Shcherbakova DM. J Mol Biol. 2023 Dec 15;435(24):168360. doi: 10.1016/j.jmb.2023.168360. Epub 2023 Nov 8. 10.1016/j.jmb.2023.168360 PubMed 37949312
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