NIR-AG
(Plasmid #210728)

• Purpose: NIR light-activated GSDMD
• Depositing Lab: Vladislav Verkhusha
• Publication: Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360. doi: 10.1016/j.jmb.2023.168360. Epub 2023 Nov 8.

Backbone

• Vector backbone: pN1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3813
• Vector type: Mammalian Expression
• Selectable markers: EGFP

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NIR-AG
• Species: H. sapiens (human)
• Insert Size (bp): 6038
• Entrez Gene: GSDMD (a.k.a. DF5L, DFNA5L, FKSG10, GSDMDC1)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: MunI (unknown if destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: human GSDMD was cloned from Addgene plasmid # 111559

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry