pBabe-Sik-YF
(Plasmid #210723)

• Purpose: Construct for stable expression of constitutively active mouse Ptk6 (Sik) in mammalian cells.
• Depositing Lab: Angela Tyner
• Publication: Derry et al Mol Cell Biol. 2000 Aug;20(16):6114-26. doi: 10.1128/MCB.20.16.6114-6126.2000.

Backbone

• Vector backbone: pBabe-puro
• Backbone size w/o insert (bp): 5061
• Total vector size (bp): 6413
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Protein tyrosine kinase 6
• Alt name: Ptk6
• Alt name: Sik
• Species: M. musculus (mouse)
• Insert Size (bp): 1352
• Mutation: Tyrosine 447 changed to Phenylalanine
• GenBank ID: NP_033210.1

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry