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Addgene

pBabe-Sik-YF
(Plasmid #210723)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210723 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabe-puro
  • Backbone size w/o insert (bp) 5061
  • Total vector size (bp) 6413
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Protein tyrosine kinase 6
  • Alt name
    Ptk6
  • Alt name
    Sik
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1352
  • Mutation
    Tyrosine 447 changed to Phenylalanine
  • GenBank ID
    NP_033210.1
  • Entrez Gene
    Ptk6 (a.k.a. BRK, Sik, Tksk, tks)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe-Sik-YF was a gift from Angela Tyner (Addgene plasmid # 210723 ; http://n2t.net/addgene:210723 ; RRID:Addgene_210723)

  • For your References section:

    Sik (BRK) phosphorylates Sam68 in the nucleus and negatively regulates its RNA binding ability. Derry JJ, Richard S, Valderrama Carvajal H, Ye X, Vasioukhin V, Cochrane AW, Chen T, Tyner AL. Mol Cell Biol. 2000 Aug;20(16):6114-26. doi: 10.1128/MCB.20.16.6114-6126.2000. 10.1128/MCB.20.16.6114-6126.2000 PubMed 10913193
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