pBabe-PTK6-YF
(Plasmid
#210715)
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PurposeConstruct for stable expression of constitutively active human PTK6 in mammalian cells.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 210715 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBabe-puro
- Backbone size w/o insert (bp) 5874
- Total vector size (bp) 7214
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameProtein tyrosine kinase 6
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Alt namePTK6
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Alt nameBRK
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SpeciesH. sapiens (human)
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Insert Size (bp)1340
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MutationTyrosine 447 changed to Phenylalanine
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GenBank IDNP_005966.1
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBabe-PTK6-YF was a gift from Angela Tyner (Addgene plasmid # 210715 ; http://n2t.net/addgene:210715 ; RRID:Addgene_210715) -
For your References section:
Protein tyrosine kinase 6 directly phosphorylates AKT and promotes AKT activation in response to epidermal growth factor. Zheng Y, Peng M, Wang Z, Asara JM, Tyner AL. Mol Cell Biol. 2010 Sep;30(17):4280-92. doi: 10.1128/MCB.00024-10. Epub 2010 Jul 6. 10.1128/MCB.00024-10 PubMed 20606012
Map uploaded by the depositor.
