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Addgene

pBabe-PTK6-YF
(Plasmid #210715)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210715 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBabe-puro
  • Backbone size w/o insert (bp) 5874
  • Total vector size (bp) 7214
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Protein tyrosine kinase 6
  • Alt name
    PTK6
  • Alt name
    BRK
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1340
  • Mutation
    Tyrosine 447 changed to Phenylalanine
  • GenBank ID
    NP_005966.1
  • Entrez Gene
    PTK6 (a.k.a. BRK)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe-PTK6-YF was a gift from Angela Tyner (Addgene plasmid # 210715 ; http://n2t.net/addgene:210715 ; RRID:Addgene_210715)
  • For your References section:

    Protein tyrosine kinase 6 directly phosphorylates AKT and promotes AKT activation in response to epidermal growth factor. Zheng Y, Peng M, Wang Z, Asara JM, Tyner AL. Mol Cell Biol. 2010 Sep;30(17):4280-92. doi: 10.1128/MCB.00024-10. Epub 2010 Jul 6. 10.1128/MCB.00024-10 PubMed 20606012