UIM-ECFP
(Plasmid #21067)

• Depositing Lab: Xiaowei Zhuang
• Publication: Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 105(33):11790-5.

Backbone

• Vector backbone: pECFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4733
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: UIM
• Alt name: Epsin1
• Species: R. norvegicus (rat)
• Insert Size (bp): 217
• GenBank ID: NC_005100
• Entrez Gene: Epn1
• Tag / Fusion Protein:
  • ECFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: AgeI (unknown if destroyed)
• 5′ sequencing primer: CMV-Forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gift of P. De Camilli (Yale University, New Haven, CT) for providing plasmids containing rat epsin 1 cDNA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry