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pTT3-ecdCD86-ST3-His
(Plasmid #210664)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210664 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTT3
  • Backbone size w/o insert (bp) 6050
  • Total vector size (bp) 6761
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Extracellular domain of human CD86 fused to Spytag003 and Histag
  • Alt name
    CD86
  • Alt name
    B7-2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    711
  • GenBank ID
    AAH40261.1
  • Entrez Gene
    CD86 (a.k.a. B7-2, B7.2, B70, CD28LG2, LAB72)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Spytag003 (C terminal on insert)
    • Histag (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GCTCCTCTGAAGATTCAAGCTTATTTCAATG
  • 3′ sequencing primer ATGGTGATGGTGATGATGTTTATACCTCTTAT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTT3-ecdCD86-ST3-His was a gift from Omer Dushek (Addgene plasmid # 210664 ; http://n2t.net/addgene:210664 ; RRID:Addgene_210664)

  • For your References section:

    Using CombiCells, a platform for titration and combinatorial display of cell surface ligands, to study T-cell antigen sensitivity modulation by accessory receptors. Patel A, Andre V, Eguiguren SB, Barton MI, Burton J, Denham EM, Pettmann J, Morch AM, Kutuzov MA, Siller-Farfan JA, Dustin ML, van der Merwe PA, Dushek O. EMBO J. 2024 Jan;43(1):132-150. doi: 10.1038/s44318-023-00012-1. Epub 2023 Dec 18. 10.1038/s44318-023-00012-1 PubMed 38177315
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