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Addgene

pJL1-FETCH1-StrepII
(Plasmid #210648)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210648 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJL1
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FETCH1
  • Species
    Synthetic
  • Promoter T7
  • Tag / Fusion Protein
    • Strep-TagII (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gibson cloning used NdeI (5') and SalI (3') sites

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJL1-FETCH1-StrepII was a gift from Michael Jewett (Addgene plasmid # 210648 ; http://n2t.net/addgene:210648 ; RRID:Addgene_210648)

  • For your References section:

    Cell-Free Translation Quantification via a Fluorescent Minihelix. Willi JA, Karim AS, Jewett MC. ACS Synth Biol. 2024 Jul 19;13(7):2253-2259. doi: 10.1021/acssynbio.4c00266. Epub 2024 Jul 9. 10.1021/acssynbio.4c00266 PubMed 38979618
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