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Addgene

pEK24
(Plasmid #210597)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210597 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGI3EM17
  • Backbone manufacturer
    Edgar Medina
  • Backbone size w/o insert (bp) 9373
  • Total vector size (bp) 12156
  • Vector type
    Bacterial Expression, Yeast Expression, Luciferase
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BdACTINpro-Hshph-G4S-nanoluc-ScADH1ter
  • Species
    S. cerevisiae (budding yeast); Batrachochytrium dendrobatidis, Oplophorus gracilirostris
  • Insert Size (bp)
    2783
  • Promoter BdACTINpro
  • Tag / Fusion Protein
    • NanoLuc luciferase (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer gtaaaacgacggccagt
  • 3′ sequencing primer caggaaacagctatgac
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.10.17.561934 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEK24 was a gift from Lillian Fritz-Laylin (Addgene plasmid # 210597 ; http://n2t.net/addgene:210597 ; RRID:Addgene_210597)

  • For your References section:

    Genetic transformation of the frog-killing chytrid fungus Batrachochytrium dendrobatidis. Kalinka E, Brody SM, Swafford AJM, Medina EM, Fritz-Laylin LK. Proc Natl Acad Sci U S A. 2024 Jan 23;121(4):e2317928121. doi: 10.1073/pnas.2317928121. Epub 2024 Jan 18. 10.1073/pnas.2317928121 PubMed 38236738
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