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pUB_SM-FLuc0_EMCV-Ren
(Plasmid #210590)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210590 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUB
  • Total vector size (bp) 12075
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Spaghetti monster (FLAG)-nonoptimal FLuc
  • Alt name
    Firefly
  • Alt name
    FLuc
  • Insert Size (bp)
    2661
  • Promoter UBC human
  • Tag / Fusion Protein
    • Spaghetti monster (FLAG) (N terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Optimal Renilla luciferase
  • Alt name
    Renilla
  • Alt name
    RLuc
  • Insert Size (bp)
    936
  • Promoter EMCV IRES

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TGGCTCTCCTCAAGCGTATT
  • 3′ sequencing primer CGCAAGTTAGGTTTTGTCAAAGAAAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUB_SM-FLuc0_EMCV-Ren was a gift from Olivia Rissland (Addgene plasmid # 210590 ; http://n2t.net/addgene:210590 ; RRID:Addgene_210590)

  • For your References section:

    Synonymous codon usage regulates translation initiation. Barrington CL, Galindo G, Koch AL, Horton ER, Morrison EJ, Tisa S, Stasevich TJ, Rissland OS. Cell Rep. 2023 Dec 26;42(12):113413. doi: 10.1016/j.celrep.2023.113413. Epub 2023 Dec 12. 10.1016/j.celrep.2023.113413 PubMed 38096059
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