PGRP-LCa
(Plasmid
#21059)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21059 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFastBacHT A
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4856
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePGRP-LCa
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SpeciesD. melanogaster (fly)
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Insert Size (bp)510
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Entrez GenePGRP-LF (a.k.a. Dmel_CG4437, CG4437, Dmel\CG4437, PGRP-like, PGRPLF, anon-WO0118547.581, pgrp-lf)
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer polyhedrin forward
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PGRP-LCa was a gift from Johann Deisenhofer (Addgene plasmid # 21059 ; http://n2t.net/addgene:21059 ; RRID:Addgene_21059) -
For your References section:
Structure of the ectodomain of Drosophila peptidoglycan-recognition protein LCa suggests a molecular mechanism for pattern recognition. Chang CI, Ihara K, Chelliah Y, Mengin-Lecreulx D, Wakatsuki S, Deisenhofer J. Proc Natl Acad Sci U S A. 2005 Jul 19. 102(29):10279-84. 10.1073/pnas.0504547102 PubMed 16006509