PGRP-LCa
(Plasmid #21059)

• Depositing Lab: Johann Deisenhofer
• Publication: Chang et al Proc Natl Acad Sci U S A. 2005 Jul 19. 102(29):10279-84.

Backbone

• Vector backbone: pFastBacHT A
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4856
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PGRP-LCa
• Species: D. melanogaster (fly)
• Insert Size (bp): 510
• Entrez Gene: PGRP-LF (a.k.a. Dmel_CG4437, CG4437, Dmel\CG4437, PGRP-like, PGRPLF, anon-WO0118547.581, pgrp-lf)
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: polyhedrin forward
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry