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Addgene

PGRP-LCx
(Plasmid #21058)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21058 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBacHT A with mellitin seq
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4856
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PGRP-LCx
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    507
  • Entrez Gene
    PGRP-LC (a.k.a. Dmel_CG4432, (PGRP)-LC, CG4432, Dm PGRP-LC, Dmel\CG4432, PGRP-LCa, PGRP-LCd, PGRP-LCx, PGRPLC, Pgrp-LC, ird7, pgrp, pgrp lc, pgrp-lc, tot)
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer polyhedrin forward
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PGRP-LCx was a gift from Johann Deisenhofer (Addgene plasmid # 21058 ; http://n2t.net/addgene:21058 ; RRID:Addgene_21058)

  • For your References section:

    Structure of tracheal cytotoxin in complex with a heterodimeric pattern-recognition receptor. Chang CI, Chelliah Y, Borek D, Mengin-Lecreulx D, Deisenhofer J. Science. 2006 Mar 24. 311(5768):1761-4. 10.1126/science.1123056 PubMed 16556841
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