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pUB_NonoptλHAramp-FLuc100
(Plasmid #210579)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210579 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUB
  • Total vector size (bp) 9812
  • Vector type
    Mammalian Expression, Lentiviral, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nonoptimal lambda HA-Optimal Firefly luciferase
  • Alt name
    Firefly
  • Alt name
    FLuc
  • Insert Size (bp)
    1840
  • Promoter UBC human
  • Tag / Fusion Protein
    • lambda HA (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer UBC_F
  • 3′ sequencing primer CGCAAGTTAGGTTTTGTCAAAGAAAG
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Lambda HA tag synthesized by Genscript

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUB_NonoptλHAramp-FLuc100 was a gift from Olivia Rissland (Addgene plasmid # 210579 ; http://n2t.net/addgene:210579 ; RRID:Addgene_210579)

  • For your References section:

    Synonymous codon usage regulates translation initiation. Barrington CL, Galindo G, Koch AL, Horton ER, Morrison EJ, Tisa S, Stasevich TJ, Rissland OS. Cell Rep. 2023 Dec 26;42(12):113413. doi: 10.1016/j.celrep.2023.113413. Epub 2023 Dec 12. 10.1016/j.celrep.2023.113413 PubMed 38096059
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