ipGL2Cas
(Plasmid
#210555)
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PurposeBinary vector expressing the estradiol-inducible Lineage Tracing system. XVE expression driven by GL2 promoter.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 210555 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSUN
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Vector typePlant Expression
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Selectable markersSeed Venus Fluorescence
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGL2p::XVE::UBQ10t::LexA::SpCas9::PEA3AT::VenusSSM
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SpeciesOther
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ipGL2Cas was a gift from Ortrun Mittelsten Scheid (Addgene plasmid # 210555 ; http://n2t.net/addgene:210555 ; RRID:Addgene_210555) -
For your References section:
A versatile CRISPR-based system for lineage tracing in living plants. Dona M, Bradamante G, Bogojevic Z, Gutzat R, Streubel S, Mosiolek M, Dolan L, Mittelsten Scheid O. Plant J. 2023 Jul 5. doi: 10.1111/tpj.16378. 10.1111/tpj.16378 PubMed 37403571