LT1
(Plasmid
#210552)
-
PurposeBinary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT1 reporter
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 210552 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepSUN
-
Vector typePlant Expression
-
Selectable markersSeed RFP fluorescence
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namepU6::AAVS1gRNA::LT1::mCherrySSM
-
SpeciesOther
Cloning Information
- Cloning method Unknown
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
LT1 was a gift from Ortrun Mittelsten Scheid (Addgene plasmid # 210552 ; http://n2t.net/addgene:210552 ; RRID:Addgene_210552) -
For your References section:
A versatile CRISPR-based system for lineage tracing in living plants. Dona M, Bradamante G, Bogojevic Z, Gutzat R, Streubel S, Mosiolek M, Dolan L, Mittelsten Scheid O. Plant J. 2023 Jul 5. doi: 10.1111/tpj.16378. 10.1111/tpj.16378 PubMed 37403571
