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Addgene

PGRP-SA
(Plasmid #21055)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21055 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Pfastbac1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4775
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PGRP-SA
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    612
  • Entrez Gene
    PGRP-SA (a.k.a. Dmel_CG11709, BcDNA:AT30827, CG11709, Dm PGRP-SA, Dmel\CG11709, SA, SEMMELWEIS, pgrp-sa, seml)
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer polyhedrin forward
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PGRP-SA was a gift from Johann Deisenhofer (Addgene plasmid # 21055 ; http://n2t.net/addgene:21055 ; RRID:Addgene_21055)

  • For your References section:

    A Drosophila pattern recognition receptor contains a peptidoglycan docking groove and unusual L,D-carboxypeptidase activity. Chang CI, Pili-Floury S, Herve M, Parquet C, Chelliah Y, Lemaitre B, Mengin-Lecreulx D, Deisenhofer J. PLoS Biol. 2004 Sep . 2(9):E277. 10.1371/journal.pbio.0020277 PubMed 15361936
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