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Addgene

pCLV3Cas-LT1
(Plasmid #210549)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210549 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSUN
  • Vector type
    Plant Expression
  • Selectable markers
    Seed RFP fluorescence

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT1::mCherrySSM
  • Species
    Other
  • Mutation
    2bp insertion after AAVS1 target sequence that disrupt in frame translation of H2B:mClover

Cloning Information

  • Cloning method Unknown

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCLV3Cas-LT1 was a gift from Ortrun Mittelsten Scheid (Addgene plasmid # 210549 ; http://n2t.net/addgene:210549 ; RRID:Addgene_210549)

  • For your References section:

    A versatile CRISPR-based system for lineage tracing in living plants. Dona M, Bradamante G, Bogojevic Z, Gutzat R, Streubel S, Mosiolek M, Dolan L, Mittelsten Scheid O. Plant J. 2023 Jul 5. doi: 10.1111/tpj.16378. 10.1111/tpj.16378 PubMed 37403571
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